Expression of CD40-CD40L during rat myocardial ischemia-reperfusion injury

AIM:To investigate the expression of CD40 and CD40 ligand(CD40L) during myocardial ischemia/reperfusion injury in rats.METHODS:In the rat modal with myocardial ischemia/reperfusion(MI/R),the changes of CD40 and CD40L expression in blood and myocardial tissues were detected by flow cytometry and immunohistochemistry, respectively. There were seven animal groups in the study, including the normal group (n=3), the ischemia 30 min group(I_{30 min},n=6),the ischemia/reperfusion 1 min,5 min,10 min,20 min and 30 min groups(each group, n=6).RESULTS:The indexes of the expression of CD40 and CD40L in I_{30 min} group were higher than those in control group,(P＜0.05); the indexes of R_{5 min},R_{10 min} groups were higher than those in the I_{30 min} group (P＜0.05);the indexes were the highest in R_{5 min} and then decreased in R_{10 min},R_{20 min},R_{30 min} group. The immunohistochemical results revealed that. CD40-CD40L expression increased on the cardiocyte membraneduring the ischemia/reperfusion injury.CONCLUSION:CD40 and CD40L may participate in the development of myocardial ischemia/reperfusion injury.     

Effects of nicotine on activation of PMNs and the ICAM-1 mRNA expression of HUVEC

AIM: To investigate the effects of nicotine on activation of PMNs, adhesion of PMNs-HUVEC and expression of ICAM-1 mRNA in HUVEC. METHODS: Activation of PMNs was measured by detecting the activity of β-glucuronidase and lysozym of PMNs. Adhesion of PMNs and HUVEC was observed. Northern blot was conducted for quantitating ICAM-1 mRNA. RESULTS: Nicotine could increase the activity of β-g [(8.76± 1.01)μg/10⁷·h vs(14.87±2.00)μg/10⁷·h,P<0.05]and Lysozym [(20.0±1.5)μg/10⁷·h vs(36.5±4.4)μg/10⁷·h,P<0.05], and also could promote adhesion of PMNs-HUVEC(38.5±9.8 vs 61.0±4.4,P＜0.05). The expression of ICAM-1 mRNA was induced by nicotine in dose-dependent fashion (10^-5-10^-3mol/L).After a 2 h treatment of HUVEC with nicontine(10^-4mol/L), the level of ICAM-1 mRNA is above the control(1.23 vs 1.63) and the highest level (2.03) is at a 12 h treatment. 764-3 can obviously counteract the above effect of nicotine. CONCLUSIONS: Nicotine could activate PMNs, enhance adhesion of PMNs-HUVEC and increase the expression of ICAM-1 mRNA in HUVEC.     

Isolation and Assessment of the in Vitro Anti-Tumor Activity of Smenothiazole A and B,Chlorinated Thiazole-Containing Peptide/Polyketides from the Caribbean Sponge,Smenospongia aurea

The study of the secondary metabolites contained in the organic extract of Caribbean sponge Smenospongia aurea led to the isolation of smenothiazole A (3) and B (4), hybrid peptide/polyketide compounds. Assays performed using four solid tumor cell lines showed that smenothiazoles exert a potent cytotoxic activity at nanomolar levels, with selectivity over ovarian cancer cells and a pro-apoptotic mechanism.     

BABA,BTH对番茄白粉病的抗性研究

分别以500 mg·L-1BABA和50 mg·L-1BTH两种诱导剂处理番茄幼苗,后期进行白粉病菌的接种,接种时间分别为诱导剂处理后1,2,3,5,8和15 d。通过病情指数、相对防效、H2O2和NO含量以及抗白粉病相关基因表达水平的测定,比较分析两种诱导剂的作用时间、最优处理模式以及抗病的相关机制。结果表明：BABA处理后防治效果最佳的间隔时间为第2天,且有效持续时间为10 d左右,BTH处理后1,3和5 d均为最佳期间且有效持续时间可达15 d,相对BABA持续期较长;BABA和BTH诱导后H2O2和NO含量均有显著增加;两种诱导剂处理后抗白粉病相关基因CHI3,GLUCA,GLUCB和PR1A表达趋势相近,但不同抗病相关基因在不同处理时间以及不同材料中均存在差异;喷施BABA和BTH后均有助于番茄抵抗白粉病菌的侵染,提高番茄的抗病能力。     

5 种呋喃香豆素分子潜在风险分析

【目的】研究 5 种呋喃香豆素分子与 DNA 的相互作用,分析其潜在风险。【方法】通过共振散 射光谱法（RLS）计算呋喃香豆素分子与 DNA 的结合饱和值（DNA BSV）,通过 DNA BSV 反映药物分子与 DNA 结合能力以评价其潜在风险。【结果】大肠杆菌 DNA 回收试验结果表明,呋喃香豆素类分子可以或部分 进入大肠杆菌细胞内,并与 DNA 发生相互作用;呋喃香豆素 RLS 信号变化与溴化乙锭十分相似,其进入大肠 杆菌 DNA 的能力由强到弱依次为：异补骨脂素 / 补骨脂素、花椒毒酚、香柠檬烯、花椒毒素。【结论】呋喃香 豆素类分子能够从体外转移到大肠杆菌内部并于其 DNA 发生相互作用,且结合模式可能是嵌入;通过计算各 自的 DNA BSV,推测 5 种呋喃香豆素类化合物潜在风险不大,但仍具有一定风险。     

枯草芽胞杆菌信号分子的分离纯化

为了获得较高纯度的B. subtilis分泌的信号分子,采用离子交换层析的方法对其进行分离纯化。本文研究了洗脱液及其pH和流速对分离纯化效果的影响,得到最佳层析条件：离子交换层析洗脱液(B液)为pH 4.0的NaAc + NaCl溶液,平衡液(A液)为pH 4.0的NaAc溶液,洗脱流速为2 mL/min;SDS-PAGE验证表明在5 kDa左右出现单个条带,且抑菌试验结果表明层析液中的B. subtilis信号分子能促进L. paracasei HD1.7产细菌素。     

骨胶原蛋白-壳聚糖共混膜中分子间作用红外光谱分析

本研究旨在利用二维红外相关光谱探讨共混膜中骨胶原蛋白和壳聚糖分子间相互作用。采用分峰拟合和二维相关分析法分析了不同混合比例共混膜的红外光谱特征。结果表明,随着壳聚糖添加量增大,骨胶原蛋白的C=O键构象首先变化,二级结构伸展,基团暴露;壳聚糖的脱乙酰化程度较高,糖基骨架C–O–C键的二维红外相关峰表明成膜过程中壳聚糖的空间构象发生改变。添加壳聚糖后,N-H键振动光谱变化十分显著;胶原蛋白的-OH、-NH2、-COOH与壳聚糖的-NH2和-COOH等基团形成分子间氢键;壳聚糖侧链-NH2质子化形成-NH3+后与胶原蛋白形成静电作用。研究表明,胶原蛋白与壳聚糖分子间形成氢键和静电相互作用,为进一步从分子层面上研究分子间相互作用提供参考。     

Inhibiting role of polydatin to expression of intercellular adhesion molecule-1 induced by lipopolysaccharide on vascular endothelial cells

AIM and METHODS:To investigate expression of intercellular adhesion molecule-1(ICAM-1) on human umbilical vein endothelial cells (HUVEC) induced by lipopolysaccharide (LPS) , and inhibiting role of polydatin by cellular immune fluorescent staining and laser confocal microscope scanning technology. RESULTS: Compared with basic expression of ICAM-1 on HUVEC, the ICAM-1 expression was enhanced significantly after stimulated by LPS from 8 h to 36 h, dose-dependent relation appeared between expression of ICAM-1 and LPS. ICAM-1 expression on endothelial cells treated only by polydatin had no abvious change,but inducing role of LPS to expression of ICAM-1 was inhibited significantly by polydatin pretreating endothelial cells. CONCLUSION:The expression of ICAM-1 on endothelial cells can be promoted by LPS , and polydatin can inhibit LPS-induced ICAM-1 expression.     

Effect of intestinal endotoxemia on ICAM-1 expression in the liver of rats

AIM: To investigate the effect of intestinal endotoxemia on intercellular adhesion molecule-1(ICAM-1) expression in the hepatic tissue.METHODS:Rat intestinal endotoxemia was induced by thioacetamide(TAA).Changes in ICAM-1 protein in the liver were detected by Western blot. RESULTS: The molecular weight of the ICAM-1 is 95 kD.Western blot analysis of hepatic tissue from control rats and rats injected with TAA within 6 hours revealed low ICMA-1 expression. ICAM-1 expression upregulation occured in rats with intestinal endotoxemia in experimental liver injury induced by TAA 12 h after TAA injection. ICAM-1 expression, plasma endotoxin level and alanine transaminase activity is of equal rank.CONCLUSION: Intestinal endotoxemia can upregulate ICAM-1 expression in the hepatic tissue and the latter is related to liver injury.     

冠心病患者血浆中分子物质和过氧化脂质水平的变化

本文检测113例冠心病患者血浆中分子物质和过氧化脂质水平,结果显示:劳累性心绞痛患者血浆中分子物质水平显著升高(P<0.05),血浆过氧化脂质水平显著降低(P<0.05);自发性心绞痛和缺血性心脏病的心力衰竭、急性心肌梗塞患者血浆中分子物质和过氧化脂质水平都显著升高(P<0.05),尤以急性心肌梗塞患者血浆中分子物质和过氧化脂质水平升高更为显著(P<0.05).本文结果表明,冠心病患者血浆中分子物质和过氧化脂质水平的变化与病变程度有关,有助于冠心病病情和预后的判断.